| Cat.No. : |
ESR1-1390H |
| Product Overview : |
Recombinant Human ESR1 Protein (10-595 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Transcription. Protein Description: Partial. |
| Description : |
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response elent (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response elent of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elents; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates mbrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. |
| Source : |
Yeast |
| Species : |
Human |
| Tag : |
His |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
67.2 kDa |
| Protein length : |
10-595 aa |
| AA Sequence : |
SGMALLHQIQ GNELEPLNRP QLKIPLERPL GEVYLDSSKP AVYNYPEGAA YEFNAAAAAN AQVYGQTGLP YGPGSEAAAF GSNGLGGFPP LNSVSPSPLM LLHPPPQLSP FLQPHGQQVP YYLENEPSGY TVREAGPPAF YRPNSDNRRQ GGRERLASTN DKGSMAMESA KETRYCAVCN DYASGYHYGV WSCEGCKAFF KRSIQGHNDY MCPATNQCTI DKNRRKSCQA CRLRKCYEVG MMKGGIRKDR RGGRMLKHKR QRDDGEGRGE VGSAGDMRAA NLWPSPLMIK RSKKNSLALS LTADQMVSAL LDAEPPILYS EYDPTRPFSE ASMMGLLTNL ADRELVHMIN WAKRVPGFVD LTLHDQVHLL ECAWLEILMI GLVWRSMEHP GKLLFAPNLL LDRNQGKCVE GMVEIFDMLL ATSSRFRMMN LQGEEFVCLK SIILLNSGVY TFLSSTLKSL EEKDHIHRVL DKITDTLIHL MAKAGLTLQQ QHQRLAQLLL ILSHIRHMSN KGMEHLYSMK CKNVVPLYDL LLEMLDAHRL HAPTSRGGAS VEETDQSHLA TAGSTSSHSL QKYYITGEAE GFPATV |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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