Recombinant Human EXO5 Protein (1-373 aa), His-SUMO-tagged

• Cat.No.: EXO5-1119H
• Product Overview: Recombinant Human EXO5 Protein (1-373 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Full Length.

Specification

• Description: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 57.8 kDa
• Protein length: 1-373 aa
• AA Sequence: MAETREEETVSAEASGFSDLSDSEF LEFLDLEDAQESKALVNMPGPSSES LGKDDKPISLQNWKRGLDILSPMER FHLKYLYVTDLATQNWCELQTAYGK ELPGFLAPEKAAVLDTGASIHLARE LELHDLVTVPVTTKEDAWAIKFLNI LLLIPTLQSEGHIREFPVFGEGEGV LLVGVIDELHYTAKGELELAELKTR RRPMLPLEAQKKKDCFQVSLYKYIF DAMVQGKVTPASLIHHTKLCLEKPL GPSVLRHAQQGGFSVKSLGDLMELV FLSLTLSDLPVIDILKIEYIHQETA TVLGTEIVAFKEKEVRAKVQHYMAY WMGHREPQGVDVEEAWKCRTCTYAD ICEWRKGSGVLSSTLAPQVKKAK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: EXO5 exonuclease 5 [ Homo sapiens (human) ]
• Official Symbol: EXO5
• Synonyms: DEM1; Exo V; hExo5; C1orf176
• Gene ID: 64789
• UniProt ID: Q9H790