Recombinant Human F10 protein, His-tagged

• Cat.No.: F10-289H
• Product Overview: Recombinant Human F10 protein(NP_000495.1) (Met 1-Lys 488) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-488 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol.
• Molecular Mass: The recombinant human coagulation factor X consists of 468 amino acids and predicts a molecular mass of 52.8 kDa. As a result of glycosylation, it migrates with the apparent molecular mass of approximately 48 and 22 kDa corresponding to the cleaved light and heavy chain respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000495.1
• Unit ID: P00742
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P00742

Gene Information

• Gene Name: F10 coagulation factor X [ Homo sapiens ]
• Official Symbol: F10
• Synonyms: F10; coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor; FX; FXA
• Gene ID: 2159
• mRNA Refseq: NM_000504
• protein Refseq: NP_000495
• MIM: 613872