Active Recombinant Human F11R, His-tagged

• Cat.No.: F11R-790H
• Product Overview: Active Recombinant Human F11R(Met 1-Ala 242)(NP_058642.1) precursor was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-242 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 10^4 cells/well are added to JAM-A-Fc coated plates (2.5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37°C.
• Molecular Mass: The secreted recombinant human JAM-A comprises 227 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, it migrates with the molecular mass of approximately 28-32 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: F11R F11 receptor [ Homo sapiens ]
• Official Symbol: F11R
• Synonyms: F11R; F11 receptor; JAM1, junctional adhesion molecule 1; junctional adhesion molecule A; CD321; JAM 1; JAM A; JAMA; JCAM; PAM 1; platelet F11 receptor; platelet adhesion molecule 1; junctional adhesion molecule 1; JAM; KAT; JAM1; PAM-1
• Gene ID: 50848
• mRNA Refseq: NM_016946
• Protein Refseq: NP_058642
• MIM: 605721
• UniProt ID: Q9Y624