Active Recombinant Human F3 protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: F3-2892H
• Product Overview: Biotinylated Recombinant Human F3 protein(P13726-1)(33-251 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 33-251 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: F3 coagulation factor III (thromboplastin, tissue factor) [ Homo sapiens ]
• Official Symbol: F3
• Synonyms: F3; coagulation factor III (thromboplastin, tissue factor); tissue factor; CD142; TF; TFA; FLJ17960
• Gene ID: 2152
• mRNA Refseq: NM_001178096
• Protein Refseq: NP_001171567
• MIM: 134390
• UniProt ID: P13726