Recombinant Human F8, His-tagged

• Cat.No.: F8-12626H
• Product Overview: Recombinant Human F8 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: C-term-208a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: F8 coagulation factor VIII, procoagulant component [ Homo sapiens ]
• Official Symbol: F8
• Synonyms: F8; coagulation factor VIII, procoagulant component; F8C; coagulation factor VIII; DXS1253E; Factor VIIIF8B; FVIII; HEMA; hemophilia A; factor VIII F8B; antihemophilic factor; coagulation factor VIIIc; AHF; F8B
• Gene ID: 2157
• mRNA Refseq: NM_000132
• Protein Refseq: NP_000123
• MIM: 300841
• UniProt ID: P00451
• Chromosome Location: Xq28
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem
• Function: copper ion binding; metal ion binding; oxidoreductase activity; protein binding

Reviews

01/04/2023

The F8 protein is of exceptional quality and has proven to be an ideal choice to meet my experimental requirements.

04/26/2019

Its purity and reliability have consistently resulted in successful outcomes in my research projects, providing me with accurate and reproducible data.

06/17/2017

With its consistent and outstanding results, the F8 protein is an indispensable tool that I highly recommend for researchers in need of reliable and precise protein analysis.

Q&As

How has technology advanced the production of F8 protein for therapeutic purposes?

Recombinant DNA technology allows for the production of F8 protein in laboratory settings, reducing the risk of contamination and increasing availability.

Can F8 protein therapy be administered at home by patients?

Many patients can self-administer F8 protein therapy at home after proper training, providing convenience and improving overall quality of life.

Are there alternative treatment approaches for hemophilia A involving F8 protein?

Gene therapy is being explored as a potential alternative, aiming to introduce functional F8 genes into patients' cells to produce the missing protein.

How does monitoring F8 protein levels contribute to hemophilia A management?

Regular monitoring helps determine the appropriate dosage for replacement therapy, ensuring that F8 levels remain within the therapeutic range.

Are there any complications associated with F8 protein replacement therapy?

In some cases, individuals may develop inhibitors—antibodies that neutralize the therapeutic F8 protein—leading to challenges in treatment.