| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Flag |
| Protein Length : |
103-281 aa |
| Description : |
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. |
| Bio-activity : |
Induces apoptosis of human Jurkat T cells at a concentration of <1 ng/mL in the presence of 0.1 to 1μg/mL TNF Ligands Enhancer. In the absence of TNF Ligands Enhancer, FasL is working at 50-100 fold higher concentrations. |
| Molecular Mass : |
~33 kDa |
| Endotoxin : |
<0.05 EU/μg purified protein by LAL test. |
| Purity : |
≥95% by SDS-PAGE |
| Storage : |
Short Term Storage: +4 centigrade
Long Term Storage: -20 centigrade
Stable for at least 6 months after receipt when stored at -20 centigrade.
Working aliquots are stable for up to 3 months when stored at -20 centigrade. |
| Concentration : |
0.1 mg/mL after reconstitution. |
| Shipping : |
Blue Ice |
| Reconstitution : |
Reconstitute with 100μL sterile water. |
| Reference : |
1. Tumor necrosis factor receptor family costimulation increases regulatory T-cell activation and function via
NF-κB: M. Lubrano di Ricco, et al.; Eur. J. Immunol. 50, 972 (2020)
2. Targeting adrenergic receptors to mitigate invariant natural killer T cells-induced acute liver injury: MB. Gon
zatti, et al.; iScience 26, 107947 (2023) |
| Storage Buffer : |
Lyophilized. Contains PBS. |
| Handling Advice : |
After reconstitution, prepare aliquots and store at -20 centigrade.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions. |
| Crossreactivity : |
Human, Mouse |
