Recombinant Human Fas Ligand soluble, FLAG-tagged

• Cat.No.: FAS-38H
• Product Overview: The extracellular domain of human FasL (sFasL, aa. 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG-tag. Glycosylation of rhsFasL is similar to natural human FasL. The recombinant protein is produced in HEK 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 103-281 a.a.
• Form: Provided as a lyophilized powder. Contains PBS.
• Molecular Mass: ~32 kDa (non-glycosylated), ~35 kDa (glycosylated) (SDS-PAGE)
• Endotoxin: Endotoxin content is<0.1 eu/μg="" purified="" protein="" as="" determined="" by="" lal="" test.="">
• Purity: ≥95% purity as determined by SDS-PAGE.
• Characteristic: rhsFasL binds to human, mouse and rat Fas.
• Applications: Induction of Apoptosis: Recombinant FasL protein induces apoptosis in Fas-sensitive cells (ED50: 50 ng/mL in A20 cells). Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells. Alone, rhsFasL kills Fas-sensitive cells at a concentration of >10 ng/mL. Use of a secondary enhancing cross-linking antibody reacting with rhsFasL increases the activity of rhsFasL approximately 50-fold.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Notes: Results using sFasL may differ from those obtained with agonistic antibodies.
• Storage: Store at -20°C long term. Once rehydrated it is recommended to prepare appropriate aliquots and store them at -20°C. Avoid repeated freeze/thaw cycles.
• Reconstitution: Prepare sterile stock solution by dissolving rhsFasL in 100 μL sterile water (0.1 mg/mL in PBS). Further dilutions should be made with medium containing 5% fetal calf serum.
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ]
• Official Symbol: FAS
• Synonyms: FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Gene ID: 355
• mRNA Refseq: NM_000043
• Protein Refseq: NP_000034
• MIM: 134637
• UniProt ID: P25445
• Chromosome Location: 10q24.1
• Pathway: Activation of Pro-Caspase 8, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Alzheimers disease, organism-specific biosystem
• Function: binding; identical protein binding; kinase binding; protein binding; receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; tumor necrosis factor-activated receptor activity