Active Recombinant Human FCGR1A protein, His-tagged

• Cat.No.: FCGR1A-2224H
• Product Overview: Active Recombinant Human FCGR1A protein(NP_000557.1) (Met 1-Pro 288) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-288 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized CD64 at 10 μg/ml can bind human IgG1 with a linear range of 0.39-25 ng/ml. 2. Scatchard analysis showed the affinity constant (Kd) of CD64 bound to Human IgG1 was 0.059 nM.
• Molecular Mass: The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
• Official Symbol: FCGR1A
• Synonyms: FCGR1A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; CD64; CD64A; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; FCRI; IGFR1; FLJ18345
• Gene ID: 2209
• mRNA Refseq: NM_000566
• Protein Refseq: NP_000557
• MIM: 146760
• UniProt ID: P12314