| Description : |
Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
77.9 kDa |
| AA Sequence : |
MALDGIRMPDGCYADGTWELSVHVT DLNRDVTLRVTGEVHIGGVMLKLVE KLDVKKDWSDHALWWEKKRTWLLKT HWTLDKYGIQADAKLQFTPQHKLLR LQLPNMKYVKVKVNFSDRVFKAVSD ICKTFNIRHPEELSLLKKPRDPTKK KKKKLDDQSEDEALELEGPLITPGS GSIYSSPGLYSKTMTPTYDAHDGSP LSPTSAWFGDSALSEGNPGILAVSQ PITSPEILAKMFKPQALLDKAKINQ GWLDSSRSLMEQDVKENEALLLRFK YYSFFDLNPKYDAIRINQLYEQAKW AILLEEIECTEEEMMMFAALQYHIN KLSIMTSENHLNNSDKEVDEVDAAL SDLEITLEGGKTSTILGDITSIPEL ADYIKVFKPKKLTLKGYKQYWCTFK DTSISCYKSKEESSGTPAHQMNLRG CEVTPDVNISGQKFNIKLLIPVAEG MNEIWLRCDNEKQYAHWMAACRLAS KGKTMADSSYNLEVQNILSFLKMQH LNPDPQLIPEQITTDITPECLVSPR YLKKYKNKQITARILEAHQNVAQMS LIEAKMRFIQAWQSLPEFGITHFIA RFQGGKKEELIGIAYNRLIRMDAST GDAIKTWRFSNMKQWNVNWEIKMVT VEFADEVRLSFICTEVDCKVVHEFI GGYIFLSTRAKDQNESLDEEMFYKL TSGWVTRTRPLEQKLISEEDLAAND ILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
