Recombinant Human FGF1, His-tagged

• Cat.No.: FGF1-26204TH
• Product Overview: Recombinant full length mature Human FGF1 with His tag produced in the endosperm tissue of barley grain ; 157 amino acids with tag, Predicted MWt 18.1 kDa.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described.
• Conjugation: HIS
• Biological activity: Bioactivity:The bioactivity was determined by dose-dependent effect of Human FGF1 on proliferation of BaF3 cells transfected with FGF receptor. ED50 for FGF1-26204TH lies in the range of 1.00-1.50 ng/ml.
• Form: Lyophilised:Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS, pH 7.2
• Storage: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
• Sequence Similarities: Belongs to the heparin-binding growth factors family.

Gene Information

• Gene Name: FGF1 fibroblast growth factor 1 (acidic) [ Homo sapiens ]
• Official Symbol: FGF1
• Synonyms: FGF1; fibroblast growth factor 1 (acidic); FGFA; heparin-binding growth factor 1; AFGF; ECGF; ECGF beta; ECGFA; ECGFB; endothelial cell growth factor; alpha; beta; FGF alpha; GLIO703; HBGF1; heparin binding growth factor 1
• Gene ID: 2246
• mRNA Refseq: NM_000800
• Protein Refseq: NP_000791
• MIM: 131220
• Uniprot ID: P05230
• Chromosome Location: 5q31.3-q33.2
• Pathway: Downstream signaling of activated FGFR, organism-specific biosystem; FGF signaling pathway, organism-specific biosystem; FGFR ligand binding and activation, organism-specific biosystem; FGFR1 ligand binding and activation, organism-specific biosystem; FGFR1b ligand binding and activation, organism-specific biosystem
• Function: S100 alpha binding; fibroblast growth factor receptor binding; growth factor activity; heparin binding; protein binding