Active Recombinant Human FGF1 protein

Species :	Human
Source :	E.coli
Tag :	Non
Protein Length :	16-155 aa
Form :	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10^6 IU/mg in the presence of 10 μg/ml of heparin.
Molecular Mass :	Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
AASequence :	MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Endotoxin :	Less than 1 EU/µg of rHuaFGF as determined by LAL method.
Purity :	> 95 % by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	FGF1 fibroblast growth factor 1 (acidic) [ Homo sapiens ]
Official Symbol	FGF1
Synonyms	FGF1; fibroblast growth factor 1 (acidic); FGFA; fibroblast growth factor 1; AFGF; ECGF; ECGF beta; ECGFA; ECGFB; endothelial cell growth factor; alpha; beta; FGF alpha; GLIO703; HBGF1; heparin binding growth factor 1; heparin-binding growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha; FGF-1; HBGF-1; ECGF-beta; FGF-alpha;
Gene ID	2246
mRNA Refseq	NM_000800
Protein Refseq	NP_000791
MIM	131220
UniProt ID	P05230