Active Recombinant Human FGF2 protein

Species :	Human
Source :	E.coli
Tag :	Non
Protein Length :	143-288 aa
Form :	Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 7.6.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10^7 U/mg.
Molecular Mass :	Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
AASequence :	MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Endotoxin :	Less than 1 EU/µg of rHubFGF as determined by LAL method.
Purity :	> 96% by SDS-PAGE analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ]
Official Symbol	FGF2
Synonyms	FGF2; fibroblast growth factor 2 (basic); FGFB; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; BFGF; FGF-2; HBGF-2;
Gene ID	2247
mRNA Refseq	NM_002006
Protein Refseq	NP_001997
MIM	134920
UniProt ID	P09038