Active Recombinant Human FGF2 Protein, Pre-aliquoted

• Cat.No.: FGF2-15H
• Product Overview: Recombinant Human FGF2 Protein, Pro143 - Ser288, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6 ng/mL.
• Molecular Mass: Predicted Molecular Mass:16.5 kDa
  SDS-PAGE: 17 kDa, reducing conditions
• Protein length: Pro143-Ser288
• N-terminal Sequence Analysis: Ala-Pro143
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 [ Homo sapiens (human) ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2; BFGF; FGFB; FGF-2; HBGF-2; fibroblast growth factor 2; basic fibroblast growth factor bFGF; fibroblast growth factor 2 (basic); heparin-binding growth factor 2; prostatropin
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• UniProt ID: P09038