| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
33-170 aa |
| Form : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : |
FGF22(Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant human FGF22 using Balb/c 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF22 diluted with serum-free standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. It was obvious that FGF22 significantly increased cell viability of 3T3 cells. The ED50 of recombinant human FGF22 is 187ng/ml. |
| Molecular Mass : |
19.9kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
