Active Recombinant Human FGF22 protein, His-tagged

• Cat.No.: FGF22-3585H
• Product Overview: Active Recombinant Human FGF22 protein(Q9HCT0)(33-170 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 33-170 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: FGF22(Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant human FGF22 using Balb/c 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF22 diluted with serum-free standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. It was obvious that FGF22 significantly increased cell viability of 3T3 cells. The ED50 of recombinant human FGF22 is 187ng/ml.
• Molecular Mass: 19.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FGF22 fibroblast growth factor 22 [ Homo sapiens ]
• Official Symbol: FGF22
• Synonyms: FGF22; fibroblast growth factor 22; FGF-22
• Gene ID: 27006
• mRNA Refseq: NM_020637
• Protein Refseq: NP_065688
• MIM: 605831
• UniProt ID: Q9HCT0