Recombinant Human FH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human FH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : FH-4851H
Product Overview : FH MS Standard C13 and N15-labeled recombinant protein (NP_000134) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 54.6 kDa
AA Sequence : MYRALRLLAR SRPLVRAPAA ALASAPGLGG AAVPSFWPPN AARMASQNSF RIEYDTFGEL KVPNDKYYGA QTVRSTMNFK IGGVTERMPT PVIKAFGILK RAAAEVNQDY GLDPKIANAI MKAADEVAEG KLNDHFPLVV WQTGSGTQTN MNVNEVISNR AIEMLGGELG SKIPVHPNDH VNKSQSSNDT FPTAMHIAAA IEVHEVLLPG LQKLHDALDA KSKEFAQIIK IGRTHTQDAV PLTLGQEFSG YVQQVKYAMT RIKAAMPRIY ELAAGGTAVG TGLNTRIGFA EKVAAKVAAL TGLPFVTAPN KFEALAAHDA LVELSGAMNT TACSLMKIAN DIRFLGSGPR SGLGELILPE NEPGSSIMPG KVNPTQCEAM TMVAAQVMGN HVAVTVGGSN GHFELNVFKP MMIKNVLHSA RLLGDASVSF TENCVVGIQA NTERINKLMN ESLMLVTALN PHIGYDKAAK IAKTAHKNGS TLKETAIELG YLTAEQFDEW VKPKDMLGPK TRTRPLEQKL ISEEDLAAND ILDYKDDDDK V
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : FH fumarate hydratase [ Homo sapiens (human) ]
Official Symbol : FH
Synonyms : FH; fumarate hydratase; fumarate hydratase, mitochondrial; fumarase; MCL; LRCC; HLRCC; MCUL1;
Gene ID : 2271
mRNA Refseq : NM_000143
Protein Refseq : NP_000134
MIM : 136850
UniProt ID : P07954

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