Recombinant Human Fibroblast Growth Factor 21

• Cat.No.: FGF21-103H
• Product Overview: Recombinant Human Fibroblast Growth Factor-21 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 181 amino acids and an N-terminal Methionin (bold), having a molecular weight of 19.7 kDa (calculated). The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence. The FGF-21 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: FGF21 is a growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.
• Amino Acid Sequence: MHPIPDS SPLLQFGGQV RQRYLYTDDA QQTEAHLEIR EDGTVGGAAD QSPESLLQLK ALKPGVIQIL GVKTSRFLCQ RPDGALYGSL HFDPEACSFR ELLLEDGYNV YQSEAHGLPL HLPGNKSPHR DPAPRGPARF LPLPGLPPAP PEPPGILAPQ PPDVGSSDPL SMVGPSQGRS PSYAS.
• Physical Appearance: Sterile Filtered white lyophilized powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20 mM TRIS, 20 mM NaCl, pH 7.5.
• Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Human Recombinant sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized FGF-21 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: FGF21 fibroblast growth factor 21 [ Homo sapiens ]
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 26291
• mRNA Refseq: NM_019113
• Protein Refseq: NP_061986
• UniProt ID: Q9NSA1
• Chromosome Location: 19q13.1-qter
• MIM: 609436
• Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: growth factor activity; protein binding