Recombinant Human FKBP10 protein, His-tagged

• Cat.No.: FKBP10-1289H
• Product Overview: Recombinant Human FKBP10 aa. (Val158~Asp440 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Val158~Asp440
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FKBP10 FK506 binding protein 10 [ Homo sapiens (human) ]
• Official Symbol: FKBP10
• Synonyms: FKBP10; FK506 binding protein 10; OI6; OI11; BRKS1; FKBP65; PPIASE; hFKBP65; peptidyl-prolyl cis-trans isomerase FKBP10; 65 kDa FK506-binding protein; 65 kDa FKBP; FK506 binding protein 10, 65 kDa; FKBP-10; FKBP-65; PPIase FKBP10; immunophilin FKBP65; rotamase
• Gene ID: 60681
• mRNA Refseq: NM_021939.3
• Protein Refseq: NP_068758.3
• UniProt ID: Q96AY3