Recombinant Human FLT3L protein FLT3LG-25H

Recombinant Human FLT3L protein


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Recombinant Human FLT3L protein

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Cat.No. : FLT3LG-25H
Product Overview : Recombinant Human FLT3L (27–181 of P49771-1 FLT3L_HUMAN) protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities.
Source : Nicotiana benthamiana
Species : Human
Form : Lyophilized from a Tris HCl 0.05M buffer pH 7.4.
Bio-activity : The activity of Flt-3 is determinated by the dose-dependent stimulation of the proliferation of human acute myeloid leukemia cells (OCMI-AML5). ED50 is typically ≤ 1 ng/ml.
Molecular Mass : It has a predicted molecular mass of 18.4 kDa, however as result of potential glycosylation, the recombinant protein could migrate with an apparent molecular mass of 18-25 kDa in SDS-PAGE.
Endotoxin : 1 EU/µg determined by LAL method
Purity : >95 % as determined by SDS-PAGE analysis
Applications : BIOASSAY, SDS-PAGE, WB.
Storage : Frozen / Solution. For extended storage, conserve at −80ºC. Repeated freezing and thawing is not recommended. Store working aliquots at 4ºC for up to one week.
Reconstitution : Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Optimal concentration should be determined for specific application and cell lines.
Gene Name : FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
Official Symbol : FLT3LG
Synonyms : FLT3LG; fms-related tyrosine kinase 3 ligand; FL; Flt3 ligand
Gene ID : 2323
mRNA Refseq : NM_001459
Protein Refseq : NP_001450
MIM : 600007
UniProt ID : P49771
Chromosome Location : 19q13.3
Pathway : Cytokine-cytokine receptor interaction; atopoietic cell lineage; Pathways in cancer
Function : cytokine activity; protein homodimerization activity; receptor tyrosine kinase binding

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