Recombinant Human FOLR1 Protein, His-Avi-tagged, Biotinylated

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Recombinant Human FOLR1 Protein, His-Avi-tagged, Biotinylated

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Cat.No. : FOLR1-031H
Product Overview : Biotinylated Human FOLR1, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Arg 25 - Met 233 (Accession # P15328-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Arg 25
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Folic acid-BSA conjugate at 5 ug/mL (100 uL/well) can bind Biotinylated Human FOLR1, His Tag, Avi Tag with a linear range of 8-125 ug/mL.
Molecular Mass : The protein has a calculated MW of 28.3 kDa. The protein migrates as 38-42 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : FOLR1 folate receptor 1 (adult) [ Homo sapiens ]
Official Symbol : FOLR1
Synonyms : FOLR1; folate receptor 1 (adult); FOLR; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18; FBP;
Gene ID : 2348
mRNA Refseq : NM_000802
Protein Refseq : NP_000793
MIM : 136430
UniProt ID : P15328

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