Recombinant Human FZD4 protein, Fc-tagged

• Cat.No.: FZD4-382H
• Product Overview: Recombinant Human FZD4 (Phe37-Glu180) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Frizzled-4 (FZD4) is also known as FzE4, CD344, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD4 contains one FZ (frizzled) domain. FZD4 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD4 interacts with MAGI3 and norrin (NDP).
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Phe37
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 42.9 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Phe37-Glu180
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FZD4
• Official Symbol: FZD4
• Synonyms: FZD4; frizzled family receptor 4; EVR1, exudative vitreoretinopathy 1, frizzled (Drosophila) homolog 4, frizzled 4, seven transmembrane spanning receptor, frizzled homolog 4 (Drosophila); frizzled-4; CD344; hFz4; frizzled homolog 4; WNT receptor frizzled-4; frizzled 4, seven transmembrane spanning receptor; EVR1; FEVR; Fz-4; FzE4; GPCR; FZD4S; MGC34390
• Gene ID: 8322
• mRNA Refseq: NM_012193
• Protein Refseq: NP_036325
• MIM: 604579
• UniProt ID: Q9ULV1