Recombinant Human FZD7 protein, Fc-tagged

• Cat.No.: FZD7-12H
• Product Overview: Recombinant Human FZD7 (Gln33-Leu185) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Frizzled-7 (FZD7) is also known as FzE3, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD7 contains one FZ (frizzled) domain. FZD7 is receptor for Wnt proteins. FZD7 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD7 interacts with MAGI3 and DVL1.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Gln33
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 43.3 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln33-Leu185
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FZD7
• Official Symbol: FZD7
• Synonyms: FZD7; frizzled family receptor 7; frizzled (Drosophila) homolog 7, frizzled 7, seven transmembrane spanning receptor, frizzled homolog 7 (Drosophila); frizzled-7; FzE3; fz-7; hFz7; frizzled homolog 7; Frizzled, drosophila, homolog of, 7; frizzled 7, seven transmembrane spanning receptor
• Gene ID: 8324
• mRNA Refseq: NM_003507
• Protein Refseq: NP_003498
• MIM: 603410
• UniProt ID: O75084