Recombinant Human FZD8 protein, His-Avi-tagged, Biotinylated

• Cat.No.: FZD8-946HB
• Product Overview: Biotinylated Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His, Avi tag at the C-terminus.

Specification

• Description: Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Molecular Mass: 25-35kDa, reducing conditions.
• Protein length: Ala28-Pro172
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: FZD8 frizzled class receptor 8 [ Homo sapiens (human) ]
• Official Symbol: FZD8
• Synonyms: frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
• Gene ID: 8325
• mRNA Refseq: NM_031866.3
• Protein Refseq: NP_114072.1
• MIM: 606146
• UniProt ID: Q9H461