Recombinant Human Gastrokine 1, His-tagged

• Cat.No.: GKN1-223H
• Product Overview: Recombinant Human Gastrokine 1 encoding the Mature Human Gastrokine-1 (Asn21-Asn185P) with 6 His tag on the N-Terminus was expressed inE.Coli.This protein was purified by Ni-NTA column. MW=20KD.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 21-185 a.a.
• Description: Gastrokine-1 is a protein that in humans is encoded by the GKN1 gene. The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa.
• Sequence: Mature human Gastrokine-1 (Asn²¹-Asn^185P)NYNINVNDDN NNAGSGQQSV SVNNEHNVAN VDNNNGWDSW NSIWDYGNGF AATRLFQKKT CIVHKMNKEV MPSIQSLDAL VKEKKLQGKG PGGPPPKGLM YSVNPNKVDD LSKFGKNIAN MCRGIPTYMA EEMQEASLFF YSGTCYTTSV LWIVDISFCG DTVEN.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100μg Mature human Gastrokine-1 (Asn21-Asn185P) in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
• Application: Cell biology, ELISA.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
• Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.

Gene Information

• Gene Name: GKN1 gastrokine 1 [ Homo sapiens ]
• Synonyms: GKN1; gastrokine 1; FOV; CA11; AMP18; BRICD1; MGC70354; foveolin; BRICHOS domain containing 1;AMP-18; Protein CA11; Gastrokine-1
• Gene ID: 56287
• mRNA Refseq: NM_019617
• Protein Refseq: NP_062563
• MIM: 606402
• UniProt ID: Q9NS71
• Chromosome Location: 2p13.3
• Function: growth factor activity; molecular_function