Recombinant Human GATA3 Protein, His-tagged

• Cat.No.: GATA3-2194H
• Product Overview: Recombinant Human GATA3 Protein (Tyr229-Gly444) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.2 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Tyr229-Gly444
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GATA3 GATA binding protein 3 [ Homo sapiens (human) ]
• Official Symbol: GATA3
• Synonyms: GATA3; GATA binding protein 3; trans-acting T-cell-specific transcription factor GATA-3; HDR; GATA-binding factor 3; HDRS; MGC2346; MGC5199; MGC5445
• Gene ID: 2625
• mRNA Refseq: NM_001002295
• Protein Refseq: NP_001002295
• MIM: 131320
• UniProt ID: P23771