Active Recombinant Human GBA3 protein, His-tagged

• Cat.No.: GBA3-174H
• Product Overview: Recombinant Human GBA3 protein(NP_066024.1)(Met1-Leu469), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Leu469
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-β-D glucopyranoside. The specific activity is >1,500 pmoles/min/μg.
• Molecular Mass: The recombinant human GBA3 consists of 480 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GBA3 glucosidase, beta, acid 3 (cytosolic) [ Homo sapiens ]
• Official Symbol: GBA3
• Synonyms: GBA3; glucosidase, beta, acid 3 (cytosolic); cytosolic beta-glucosidase; GLUC; klotho related protein; KLrP; klotho-related protein; cytosolic beta-glucosidase-like protein 1; CBGL1; MGC104276; MGC126878
• Gene ID: 57733
• mRNA Refseq: NM_001128432
• Protein Refseq: NP_001121904
• MIM: 606619
• UniProt ID: Q9H227