Recombinant Human GCAT Protein, His-tagged

• Cat.No.: GCAT-313H
• Product Overview: Recombinant Human GCAT fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. THis gene encodes the second enzyme in tHis pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of tHis gene is found on chromosome 14.
• Form: 25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl. Store at -80 centigradeentigradeentigrade. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Molecular Mass: 45.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: GCAT glycine C-acetyltransferase [ Homo sapiens ]
• Official Symbol: GCAT
• Synonyms: GCAT; glycine C-acetyltransferase; glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase); 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; 2 amino 3 ketobutyrate coenzyme A ligase; KBL; AKB ligase; aminoacetone synthase; glycine acetyltransferase; 2-amino-3-ketobutyrate-CoA ligase; MGC23053
• Gene ID: 23464
• mRNA Refseq: NM_001171690
• Protein Refseq: NP_001165161
• MIM: 607422
• UniProt ID: O75600