Description : |
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called ‘glucokinase’. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His |
Form : |
Liquid |
Bio-activity : |
Specific activity is > 2,000 pmol/min/μg. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37 centigrade. |
Molecular Mass : |
54.3 kDa (485aa) |
AA Sequence : |
MGSSHHHHHHSSGLVPRGSHMLDDR ARMEAAKKEKVEQILAEFQLQEEDL KKVMRRMQKEMDRGLRLETHEEASV KMLPTYVRSTPEGSEVGDFLSLDLG GTNFRVMLVKVGEGEEGQWSVKTKH QMYSIPEDAMTGTAEMLFDYISECI SDFLDKHQMKHKKLPLGFTFSFPVR HEDIDKGILLNWTKGFKASGAEGNN VVGLLRDAIKRRGDFEMDVVAMVND TVATMISCYYEDHQCEVGMIVGTGC NACYMEEMQNVELVEGDEGRMCVNT EWGAFGDSGELDEFLLEYDRLVDES SANPGQQLYEKLIGGKYMGELVRLV LLRLVDENLLFHGEASEQLRTRGAF ETRFVSQVESDTGDRKQIYNILSTL GLRPSTTDCDIVRRACESVSTRAAH MCSAGLAGVINRMRESRSEDVMRIT VGVDGSVYKLHPSFKERFHASVRRL TPSCEITFIESEEGSGRGAALVSAV ACKKACMLGQ |
Endotoxin : |
< 1.0 EU per 1μg of protein (determined by LAL method) |
Purity : |
> 95% by SDS-PAGE |
Notes : |
For research use only. This product is not intended or approved for human, diagnostics or veterin. |
Storage : |
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : |
1 mg/mL (determined by Bradford assay) |
Storage Buffer : |
In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |