Recombinant Human GCK Protein, His-tagged GCK-005H

Recombinant Human GCK Protein, His-tagged

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Recombinant Human GCK Protein, His-tagged

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Cat.No. : GCK-005H
Product Overview : Recombinant human Hexokinase4, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description : Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called ‘glucokinase’.
Source : E. coli
Species : Human
Tag : His
Form : Liquid
Bio-activity : Specific activity is > 2,000 pmol/min/μg. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37 centigrade.
Molecular Mass : 54.3 kDa (485aa)
AA Sequence : MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ
Endotoxin : < 1.0 EU per 1μg of protein (determined by LAL method)
Purity : > 95% by SDS-PAGE
Notes : For research use only. This product is not intended or approved for human, diagnostics or veterin.
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/mL (determined by Bradford assay)
Storage Buffer : In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
SDS-PAGE :

15% SDS-PAGE (3μg)

Gene Name : GCK glucokinase [ Homo sapiens (human) ]
Official Symbol : GCK
Synonyms : Hexokinase type IV; Glucokinase; GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3; glucokinase; ATP:D-hexose 6-phosphotransferase; HK IV; glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; hexokinase type IV; EC 2.7.1.2
Gene ID : 2645
mRNA Refseq : NM_000162
Protein Refseq : NP_000153
MIM : 138079
UniProt ID : P35557

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