Active Recombinant Human GDF15 protein, hFc-tagged, Low Endotoxin

• Cat.No.: GDF15-204H
• Product Overview: Recombinant Human GDF15 protein(Q99988)(Ala197-Ile308), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 197-308 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GFRAL, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GDF15 growth differentiation factor 15 [ Homo sapiens ]
• Official Symbol: GDF15
• Synonyms: GDF15; growth differentiation factor 15; growth/differentiation factor 15; MIC 1; MIC1; NAG 1; PDF; PLAB; prostate differentiation factor; PTGFB; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; MIC-1; NAG-1; GDF-15
• Gene ID: 9518
• mRNA Refseq: NM_004864
• Protein Refseq: NP_004855
• MIM: 605312
• UniProt ID: Q99988