Active Recombinant Human GFRA1 protein, His-tagged

• Cat.No.: GFRA1-80H
• Product Overview: Recombinant Human GFRA1 protein(Asp25-Ile426), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-426 aa
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human GDNF Protein at 5μg/mL (100 μL/well) can bind Recombinant Human GFRA1 with a linear range of 1-65.9 ng/mL.
• Molecular Mass: 45.74 KDa
• Endotoxin: < 0.01 EU/μg of the protein by LAL method
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: GFRA1 GDNF family receptor alpha 1 [ Homo sapiens ]
• Official Symbol: GFRA1
• Synonyms: GFRA1; GDNF family receptor alpha 1; GDNFRA; GDNF family receptor alpha-1; GDNFR; GFR ALPHA 1; RET1L; RETL1; TRNR1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha-1; GPI-linked anchor protein; PI-linked cell-surface accessory protein; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha; GFR-ALPHA-1; FLJ10561; FLJ31546; MGC23045; DKFZp313E1012; DKFZp686J0156
• Gene ID: 2674
• mRNA Refseq: NM_001145453
• Protein Refseq: NP_001138925
• MIM: 601496
• UniProt ID: P56159