Recombinant Human GFRA3 protein(Met1-Trp382), His&hFc-tagged

• Cat.No.: GFRA3-3242H
• Product Overview: Recombinant Human GFRa3 (NP_001487.2) (Met 1-Trp 382) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-382 a.a.
• Form: Lyophilized from sterile PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind mouse ARTN in a functional ELISA.
• Molecular Mass: The recombinant human GFRa3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 599 amino acids and predicts a molecular mass of 67.3 kDa. As a result of glycosylation, the rh GFRa3/Fc monomer migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GFRA3 GDNF family receptor alpha 3 [ Homo sapiens ]
• Official Symbol: GFRA3
• Synonyms: GFRA3; GDNF family receptor alpha 3; GDNF family receptor alpha-3; GFRa 3; GFR-alpha-3; GDNFR-alpha-3; GPI-linked receptor; GDNF receptor alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3
• Gene ID: 2676
• mRNA Refseq: NM_001496
• Protein Refseq: NP_001487
• MIM: 605710
• UniProt ID: O60609