Recombinant Human GFRAL protein, hFc-tagged

• Cat.No.: GFRAL-5733H
• Product Overview: Recombinant Human GFRAL protein(Q6UXV0)(Ser19-Glu351), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Ser19-Glu351
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GFRAL GDNF family receptor alpha like [ Homo sapiens ]
• Official Symbol: GFRAL
• Synonyms: GFRAL; GDNF family receptor alpha like; C6orf144, chromosome 6 open reading frame 144; GDNF family receptor alpha-like; bA360D14.1; GRAL; UNQ9356; IVFI9356; C6orf144
• Gene ID: 389400
• mRNA Refseq: NM_207410
• Protein Refseq: NP_997293
• UniProt ID: Q6UXV0