Recombinant Human GINS1 protein, His-tagged
Cat.No. : | GINS1-6453H |
Product Overview : | Recombinant Human GINS1 protein(1-196 aa), fused with N-terminal His tag, was expressed in E.coli. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-196 aa |
Form : | The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole. |
AASequence : | MFCEKAMELIRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSGGRSDLIPTIKFRHCSLLRNRRCTVAYLYDRLLRIRALRWEYGSILPNALRFHMAAEEMEWFNNYKRSLATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGEFEVDDGTSVLLKKNSQHFLPRWKCEQLIRQGVLEHILS |
Purity : | 85%, by SDS-PAGE with Coomassie Brilliant Blue staining. |
Storage : | Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles. |
Reconstitution : | Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution. |
Gene Name | GINS1 GINS complex subunit 1 (Psf1 homolog) [ Homo sapiens ] |
Official Symbol | GINS1 |
Synonyms | GINS1; GINS complex subunit 1 (Psf1 homolog); DNA replication complex GINS protein PSF1; KIAA0186; PSF1; partner of sld five-1; RP4-691N24.2; |
Gene ID | 9837 |
mRNA Refseq | NM_021067 |
Protein Refseq | NP_066545 |
MIM | 610608 |
UniProt ID | Q14691 |
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Not For Human Consumption!
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