Recombinant Human GLCE Protein, GST-tagged

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Recombinant Human GLCE Protein, GST-tagged

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Cat.No. : GLCE-4949H
Product Overview : Human GLCE full-length ORF ( NP_056369.1, 1 a.a. - 617 a.a.) recombinant protein with GST-tag at N-terminal.
Description : Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 96.5 kDa
AA Sequence : MRCLAARVNY KTLIIICALF TLVTVLLWNK CSSDKAIQFP RRSSSGFRVD GFEKRAAASE SNNYMNHVAK QQSEEAFPQE QQKAPPVVGG FNSNVGSKVL GLKYEEIDCL INDEHTIKGR REGNEVFLPF TWVEKYFDVY GKVVQYDGYD RFEFSHSYSK VYAQRAPYHP DGVFMSFEGY NVEVRDRVKC ISGVEGVPLS TQWGPQGYFY PIQIAQYGLS HYSKNLTEKP PHIEVYETAE DRDKNKPNDW TVPKGCFMAN VADKSRFTNV KQFIAPETSE GVSLQLGNTK DFIISFDLKF LTNGSVSVVL ETTEKNQLFT IHYVSNAQLI AFKERDIYYG IGPRTSWSTV TRDLVTDLRK GVGLSNTKAV KPTKIMPKKV VRLIAKGKGF LDNITISTTA HMAAFFAASD WLVRNQDEKG GWPIMVTRKL GEGFKSLEPG WYSAMAQGQA ISTLVRAYLL TKDHIFLNSA LRATAPYKFL SEQHGVKAVF MNKHDWYEEY PTTPSSFVLN GFMYSLIGLY DLKETAGEKL GKEARSLYER GMESLKAMLP LYDTGSGTIY DLRHFMLGIA PNLARWDYHT THINQLQLLS TIDESPVFKE FVKRWKSYLK GSRAKHN
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : GLCE glucuronic acid epimerase [ Homo sapiens ]
Official Symbol : GLCE
Synonyms : GLCE; glucuronic acid epimerase; D glucuronyl C5 epimerase, UDP glucuronic acid epimerase; D-glucuronyl C5-epimerase; heparan sulfate epimerase; HSEPI; KIAA0836; glucuronyl C5-epimerase; UDP-glucuronic acid epimerase; heparin/heparan sulfate-glucuronic acid C5-epimerase; heparin/heparan sulfate:glucuronic acid C5-epimerase;
Gene ID : 26035
mRNA Refseq : NM_015554
Protein Refseq : NP_056369
MIM : 612134
UniProt ID : O94923

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