Recombinant Human GLDC Protein, His-tagged

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Recombinant Human GLDC Protein, His-tagged

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Cat.No. : GLDC-492H
Product Overview : Recombinant Human GLDC(Thr81~Asp268) fused with His tag at N-terminal was expressed in E. coli.
Description : Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
Source : E. coli
Species : Human
Tag : His
Form : PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass : 22.7kDa
Protein length : Thr81~Asp268
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 92%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in PBS or others.
Gene Name : GLDC glycine dehydrogenase (decarboxylating) [ Homo sapiens ]
Official Symbol : GLDC
Synonyms : GLDC; glycine dehydrogenase (decarboxylating); glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavage system protein P); glycine dehydrogenase [decarboxylating], mitochondrial; GCSP; glycine cleavage system protein P; glycine decarboxylase; NKH; glycine decarboxylase P-protein; glycine cleavage system P protein; GCE; HYGN1; MGC138198; MGC138200;
Gene ID : 2731
mRNA Refseq : NM_000170
Protein Refseq : NP_000161
MIM : 238300
UniProt ID : P23378

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