Recombinant Human GLI1 protein, MYC/DDK-tagged

• Cat.No.: GLI1-312H
• Product Overview: Recombinant Human GLI1 fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Description: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 117.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Publication:
  An FDA-Approved Antifungal, Ketoconazole, and Its Novel Derivative Suppress tGLI1-Mediated Breast Cancer Brain Metastasis by Inhibiting the DNA-Binding Activity of Brain Metastasis-Promoting Transcription Factor tGLI1 (2021)

Gene Information

• Gene Name: GLI1 GLI family zinc finger 1 [ Homo sapiens ]
• Official Symbol: GLI1
• Synonyms: GLI1; GLI family zinc finger 1; GLI, glioma associated oncogene family zinc finger 1 , glioma associated oncogene homolog 1 (zinc finger protein); zinc finger protein GLI1; oncogene GLI; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); GLI
• Gene ID: 2735
• mRNA Refseq: NM_001160045
• Protein Refseq: NP_001153517
• MIM: 165220
• UniProt ID: P08151
• Chromosome Location: 12q13.2-q13.3
• Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: DNA binding; metal ion binding; protein binding; transcription regulatory region DNA binding; zinc ion binding