Active Recombinant Human GDNF protein

• Cat.No.: GDNF-338H
• Product Overview: Active Recombinant Human GDNF protein(P39905-2) (Arg 83-Ile 185) was expressed and purified in HEK293, with additional two aa (Gly& Pro) at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 83-185 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured in a cell proliferation assay using SHSY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-15 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 His Chimera. 2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA.
• Molecular Mass: The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens ]
• Official Symbol: GDNF
• Synonyms: GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF
• Gene ID: 2668
• mRNA Refseq: NM_000514
• Protein Refseq: NP_000505
• MIM: 600837
• UniProt ID: P39905