Recombinant Human Glycine Cleavage System Protein H (Aminomethyl Carrier), His-tagged

• Cat.No.: GCSH-1281H
• Product Overview: Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
• Molecular Weight: 16.4 kDa (149aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GCSH glycine cleavage system protein H (aminomethyl carrier) [ Homo sapiens ]
• Official Symbol: GCSH
• Synonyms: GCSH; glycine cleavage system protein H (aminomethyl carrier); GCE; NKH; glycine cleavage system H protein, mitochondrial; OTTHUMP00000174971; lipoic acid-containing protein; mitochondrial glycine cleavage system H-protein; Glycine cleavage system H protein, mitochondrial
• Gene ID: 2653
• mRNA Refseq: NM_004483
• Protein Refseq: NP_004474
• MIM: 238330
• UniProt ID: P23434
• Chromosome Location: 16q23.2
• Function: aminomethyltransferase activity; enzyme binding