Recombinant Human Glycogen Synthase Kinase 3 Beta, His-tagged

• Cat.No.: GSK3B-2649H
• Product Overview: Recombinant human GSK-3β was fused at the N-terminus to a His•Tag sequence and expressed in E. coli. MW = 53-56 kDa.

Specification

• Cat. No.: GSK3B-2649H
• Description: Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer"s disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90rsk, c-Jun, and CREB.
• Source: E.coli.
• Purity: ≥90% by SDS-PAGE.
• Form: Solid. Lyophilized from 0.1 M NH4CO3.
• Specific Activity: 1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5.
• Recommended Reaction Conditions: 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 12 mM MgCl2, 2 mM DTT, 100 µM Na3VO4, 200 µM ATP, 30°C.
• Solubility: Reconstitute in sterile PBS to a final concentration of 1 mg/ml.
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤ -70℃. Avoid freeze/thaw. Following reconstitution, aliquot and freeze (-70°C).

Gene Information

• Gene Name: GSK3B glycogen synthase kinase 3 beta [ Homo sapiens ]
• Synonyms: GSK3B; glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; EC 2.7.11.26; glycogen synthase kinase -3 beta; GSK3beta isoform
• Gene ID: 2932
• mRNA Refseq: NM_001146156
• Protein Refseq: NP_001139628
• MIM: 605004
• UniProt ID: P49841
• Chromosome Location: 3q13.3
• Pathway: Chemokine signaling pathway; Alzheimer"s disease; Axon guidance; B cell receptor signaling pathway; Basal cell carcinoma; Cell cycle; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Hedgehog signaling pathway; Insulin signaling pathway; Melanogenesis; Neurotrophin signaling pathway; Prostate cancer; T cell receptor signaling pathway; Wnt signaling pathway; Axon guidance; Signaling by Wnt; Signalling by NGF
• Function: ATP binding; nucleotide binding; transferase activity; glycogen synthase kinase 3 activity; protein kinase A catalytic subunit binding; tau-protein kinase activity; NF-kappaB binding; beta-catenin binding; p53 binding