Recombinant Human GMDS Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GMDS-4250H
• Product Overview: GMDS MS Standard C13 and N15-labeled recombinant protein (NP_001491) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 41.9 kDa
• AA Sequence: MAHAPARCPSARGSGDGEMGKPRNV ALITGITGQDGSYLAEFLLEKGYEV HGIVRRSSSFNTGRIEHLYKNPQAH IEGNMKLHYGDLTDSTCLVKIINEV KPTEIYNLGAQSHVKISFDLAEYTA DVDGVGTLRLLDAVKTCGLINSVKF YQASTSELYGKVQEIPQKETTPFYP RSPYGAAKLYAYWIVVNFREAYNLF AVNGILFNHESPRRGANFVTRKISR SVAKIYLGQLECFSLGNLDAKRDWG HAKDYVEAMWLMLQNDEPEDFVIAT GEVHSVREFVEKSFLHIGKTIVWEG KNENEVGRCKETGKVHVTVDLKYYR PTEVDFLQGDCTKAKQKLNWKPRVA FDELVREMVHADVELMRTNPNATRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GMDS GDP-mannose 4,6-dehydratase [ Homo sapiens (human) ]
• Official Symbol: GMDS
• Synonyms: GMDS; GDP-mannose 4,6-dehydratase; GDP-mannose 4,6 dehydratase; GMD; SDR3E1; short chain dehydrogenase/reductase family 3E; member 1; GDP-D-mannose dehydratase; short chain dehydrogenase/reductase family 3E, member 1
• Gene ID: 2762
• mRNA Refseq: NM_001500
• Protein Refseq: NP_001491
• MIM: 602884
• UniProt ID: O60547