Recombinant Human GNAQ, His-tagged

• Cat.No.: GNAQ-8728H
• Product Overview: Recombinant human GNAQ protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: This locus encodes a guanine nucleotide-binding protein. The encoded protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospolipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. A related pseudogene exists on chromosome 2.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol
• Molecular Mass: 44.5kDa (382aa)
• Protein length: 1-359aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTLESIM ACCLSEEAKE ARRINDEIER QLRRDKRDAR RELKLLLLGT GESGKSTFIK QMRIIHGSGYSDEDKRGFTK LVYQNIFTAM QAMIRAMDTL KIPYKYEHNK AHAQLVREVD VEKVSAFENP YVDAIKSLWN DPGIQECYDR RREYQLSDSTKYYLNDLDRV ADPAYLPTQQ DVLRVRVPTT GIIEYPFDLQ SVIFRMVDVG GQRSERRKWI HCFENVTSIM FLVALSEYDQ VLVESDNENRMEESKALFRT IITYPWFQNS SVILFLNKKD LLEEKIMYSH LVDYFPEYDG PQRDAQAARE FILKMFVDLN PDSDKIIYSH FTCATDTENIRFVFAAVKDT ILQLNLKEYN LV
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GNAQ guanine nucleotide binding protein (G protein), q polypeptide [ Homo sapiens (human) ]
• Official Symbol: GNAQ
• Synonyms: GNAQ; GAQ; SWS; CMC1; G-ALPHA-q; guanine nucleotide binding protein (G protein), q polypeptide; guanine nucleotide-binding protein G(q) subunit alpha; guanine nucleotide-binding protein alpha-q
• Gene ID: 2776
• mRNA Refseq: NM_002072
• Protein Refseq: NP_002063
• MIM: 600998
• UniProt ID: P50148
• Chromosome Location: 9q21
• Pathway: ADP signalling through P2Y purinoceptor 1; Adrenergic signaling in cardiomyocytes; Alzheimer's disease
• Function: G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding