Recombinant Human GNB1 Protein, His-tagged

• Cat.No.: GNB1-2209H
• Product Overview: Recombinant Human GNB1 Protein (Ser2-Asn340) with a N-His tag was expressed in E. coli.

Specification

• Description: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.2 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Ser2-Asn340
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GNB1 guanine nucleotide binding protein (G protein), beta polypeptide 1 [ Homo sapiens (human) ]
• Official Symbol: GNB1
• Synonyms: GNB1; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; transducin beta chain 1; G protein, beta-1 subunit; beta subunit, signal-transducing proteins GS/GI; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1
• Gene ID: 2782
• mRNA Refseq: NM_002074
• Protein Refseq: NP_002065
• MIM: 139380
• UniProt ID: P62873