Recombinant Human GNMT Protein, His-tagged, Bioactivity Validated

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Recombinant Human GNMT Protein, His-tagged, Bioactivity Validated

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Cat.No. : GNMT-232H
Product Overview : Recombinant Human GNMT(1-295aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.
Description : Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC).
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Bio-activity : Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Molecular Mass : 34.9 kDa (315aa), confirmed by MALDI-TOF
Protein length : 1-295aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF
SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY
DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ
TYIPCYFIHV LKRTD
Purity : > 95% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1mg/ml (determined by Absorbance at 280nm)
Figure :

Gene Name : GNMT glycine N-methyltransferase [ Homo sapiens ]
Official Symbol : GNMT
Synonyms : GNMT; glycine N-methyltransferase;
Gene ID : 27232
mRNA Refseq : NM_018960
Protein Refseq : NP_061833
MIM : 606628
UniProt ID : Q14749

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