Recombinant Human GPA33 Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: GPA33-244HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human GPA33 (Ile22-Val235) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ile22-Val235
• Description: Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 24.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• N-terminal Sequence Analysis: Ile 22
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 647

Gene Information

• Gene Name: GPA33
• Official Symbol: GPA33
• Synonyms: GPA33; glycoprotein A33 (transmembrane); cell surface A33 antigen; A33; transmembrane glycoprotein A33; MGC129986; MGC129987
• Gene ID: 10223
• mRNA Refseq: NM_005814
• Protein Refseq: NP_005805
• MIM: 602171
• UniProt ID: Q99795