Recombinant Human GPC1 protein, Fc-tagged

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Recombinant Human GPC1 protein, Fc-tagged

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Cat.No. : GPC1-624H
Product Overview : Recombinant Human GPC1 (Asp24-Ser530) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Asp24
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 82.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Asp24-Ser530
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of GPC1-624H :

Human Glypican 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of GPC1-624H :

Immobilized Human FGF basic, Tag Free at 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag with a linear range of 1-39 ng/mL (QC tested).

Gene Name : GPC1
Official Symbol : GPC1
Synonyms : GPC1; glypican 1; glypican-1; glypican; glypican proteoglycan 1; FLJ38078
Gene ID : 2817
mRNA Refseq : NM_002081
Protein Refseq : NP_002072
MIM : 600395
UniProt ID : P35052

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