Recombinant Human GPC3 Protein (25-559aa), C-His-tagged

Cat.No. : GPC3-22H
Product Overview : Recombinant human GPC3 (25-559aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Description : Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Source : Baculovirus
Species : Human
Tag : C-His
Form : Liquid
Molecular Mass : 61.8 kDa (544aa)
Protein Length : 25-559
AA Sequence : ADPQPPPPPPDATCHQVRSFFQRLQ PGLKWVPETPVPGSDLQVCLPKGPT CCSRKMEEKYQLTARLNMEQLLQSA SMELKFLIIQNAAVFQEAFEIVVRH AKNYTNAMFKNNYPSLTPQAFEFVG EFFTDVSLYILGSDINVDDMVNELF DSLFPVIYTQLMNPGLPDSALDINE CLRGARRDLKVFGNFPKLIMTQVSK SLQVTRIFLQALNLGIEVINTTDHL KFSKDCGRMLTRMWYCSYCQGLMMV KPCGGYCNVVMQGCMAGVVEIDKYW REYILSLEELVNGMYRIYDMENVLL GLFSTIHDSIQYVQKNAGKLTTTIG KLCAHSQQRQYRSAYYPEDLFIDKK VLKVAHVEHEETLSSRRRELIQKLK SFISFYSALPGYICSHSPVAENDTL CWNGQELVERYSQKAARNGMKNQFN LHELKMKGPEPVVSQIIDKLKHINQ LLRTMSMPKGRVLDKNLDEEGFESG DCGDDEDECIGGSGDGMIKVKNQLR FLAELAYDLDVDDAPGNSQQATPKD NEISTFHNLGNVHHHHHHH
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/mL (determined by absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
SDS-PAGE: 3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Gene Name : GPC3 glypican 3 [ Homo sapiens (human) ]
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; glypican-3; glypican proteoglycan 3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Gene ID : 2719
mRNA Refseq : NM_004484
Protein Refseq : NP_004475
MIM : 300037
UniProt ID : P51654

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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08/12/2020

    The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    09/18/2019

      The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

      04/10/2019

        Another crucial aspect where the manufacturer excels is their efficient supply chain management.

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