Recombinant Human GPD2 cell lysate

• Cat.No.: GPD2-733HCL

Specification

• Description: Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.
• Species: Human
• Size: 100 ug
• Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Applications: Western Blot;Immunoprecipitation

Gene Information

• Gene Name: GPD2 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) [ Homo sapiens ]
• Official Symbol: GPD2
• Synonyms: GPD2; glycerol-3-phosphate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; mtGPD; GPDH-M; mitochondrial glycerophosphate dehydrogenase; GDH2; GPDM; mGPDH
• Gene ID: 2820
• mRNA Refseq: NM_000408
• Protein Refseq: NP_000399
• MIM: 138430
• UniProt ID: P43304
• Chromosome Location: 2q24.1
• Pathway: Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; glycerol-3-phosphate shuttle, organism-specific biosystem
• Function: calcium ion binding; glycerol-3-phosphate dehydrogenase [NAD+] activity; glycerol-3-phosphate dehydrogenase activity; oxidoreductase activity; sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity