Recombinant Human GPX1 Protein, His-tagged

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Recombinant Human GPX1 Protein, His-tagged

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Cat.No. : GPX1-5307H
Product Overview : Human GPX1 (NP_000572, 1 a.a. - 203 a.a., U49C) full-length recombinant protein with His tag expressed in Escherichia coli.
Description : This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Source : E. coli
Species : Human
Tag : His
Form : Liquid
Molecular Mass : 24.2 kDa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE
Storage : Store at -20 centigrade. For long term storage store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Concentration : 0.5 mg/mL
Storage Buffer : In 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. (30% glycerol, 2 mM DTT)
Quality Control Testing :

15% SDS-PAGE Stained with Coomassie Blue

Gene Name : GPX1 glutathione peroxidase 1 [ Homo sapiens ]
Official Symbol : GPX1
Synonyms : GPX1; glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase; GPXD; GSHPX1; MGC14399; MGC88245;
Gene ID : 2876
mRNA Refseq : NM_000581
Protein Refseq : NP_000572
MIM : 138320
UniProt ID : P07203

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