Recombinant Human Grancalcin, EF-hand Calcium Binding Protein, His-tagged

• Cat.No.: GCA-4382H
• Product Overview: GCA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-217.a.) and having a molecular mass of 26.1kDa. GCA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Protein Length: 1-217 a.a.
• Description: GCA is calcium-binding protein which is particularly abundant in human neutrophils. GCA is a member of the penta EF-hand (PEF) subfamily of EF-hand proteins, who also comprises calpain, sorcin, peflin, and ALG-2. GCA undergoes essential conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, that direct the protein to hydrophobic surfaces. GCA cooperates with L-plastin, a protein known to have actin bundling activity, which suggests that GCA has a part in the regulation of neutrophils adhesion.
• Form: The GCA protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl and 20% glycerol.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered clear solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
• Pathways: Insulin signaling pathway; mTOR signaling pathway

Gene Information

• Gene Name: GCA grancalcin, EF-hand calcium binding protein [ Homo sapiens ]
• Official Symbol: GCA
• Synonyms: GCA; grancalcin, EF-hand calcium binding protein; GCL; grancalcin; OTTHUMP00000204639; OTTHUMP00000204640; grancalcin, penta-EF-hand protein; grancalcin, EF-hand calcium-binding protein
• Gene ID: 25801
• mRNA Refseq: NM_012198
• Protein Refseq: NP_036330
• MIM: 607030
• UniProt ID: P28676
• Chromosome Location: 2q37.1
• Function: calcium ion binding; protein homodimerization activity