Recombinant Human Grancalcin, EF-hand Calcium Binding Protein, His-tagged

• Cat.No.: GCA-6903H
• Product Overview: Recombinant human GCA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 26.1 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-217aa
• Description: GCA, also known as Grancalcin, is calcium-binding protein that is especially abundant in human neutrophils. It belongs to the penta EF-hand (PEF) subfamily of EF-hand proteins, which also comprises calpain, sorcin, peflin, and ALG-2. This protein undergoes important conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, which direct the protein to hydrophobic surfaces. It interacts with L-plastin, a protein known to have actin bundling activity, which suggests that GCA may play a role in the regulation of neutrophils adhesion.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl.
• Molecular Mass: 26.1 kDa (237aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAYPGYGGGF GNFS IQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GCA grancalcin, EF-hand calcium binding protein [ Homo sapiens ]
• Official Symbol: GCA
• Synonyms: GCA; grancalcin, EF-hand calcium binding protein; grancalcin, EF hand calcium binding protein; grancalcin; GCL; grancalcin, penta-EF-hand protein; grancalcin, EF-hand calcium-binding protein
• Gene ID: 25801
• mRNA Refseq: NM_012198
• Protein Refseq: NP_036330
• MIM: 607030
• UniProt ID: P28676
• Chromosome Location: 2p14-q14.3
• Function: calcium ion binding; protein homodimerization activity