Recombinant Human GTF2E1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GTF2E1-5066H
• Product Overview: GTF2E1 MS Standard C13 and N15-labeled recombinant protein (NP_005504) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 49.3 kDa
• AA Sequence: MADPDVLTEVPAALKRLAKYVIRGF YGIEHALALDILIRNSCVKEEDMLE LLKFDRKQLRSVLNNLKGDKFIKCR MRVETAADGKTTRHNYYFINYRTLV NVVKYKLDHMRRRIETDERDSTNRA SFKCPVCSSTFTDLEANQLFDPMTG TFRCTFCHTEVEEDESAMPKKDART LLARFNEQIEPIYALLRETEDVNLA YEILEPEPTEIPALKQSKDHAATTA GAASLAGGHHREAWATKGPSYEDLY TQNVVINMDDQEDLHRASLEGKSAK ERPIWLRESTVQGAYGSEDMKEGGI DMDAFQEREEGHAGPDDNEEVMRAL LIHEKKTSSAMAGSVGAAAPVTAAN GDDSESETSESDDDSPPRPAAVAVH KREEDEEEDDEFEEVADDPIVMVAG RPFSYSEVSQRPELVAQMTPEEKEA YIAMGQRMFEDLFETRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GTF2E1 general transcription factor IIE subunit 1 [ Homo sapiens (human) ]
• Official Symbol: GTF2E1
• Synonyms: GTF2E1; general transcription factor IIE, polypeptide 1, alpha 56kDa; general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD); general transcription factor IIE subunit 1; FE; TFIIE A; TFIIE-alpha; general transcription factor IIE 56 kDa subunit; transcription initiation factor IIE subunit alpha; TF2E1; TFIIE-A
• Gene ID: 2960
• mRNA Refseq: NM_005513
• Protein Refseq: NP_005504
• MIM: 189962
• UniProt ID: P29083